36 research outputs found
Multi-Physical Parameter Cross-Sectional Imaging of Quantitative Phase and Fluorescence by Integrated Multimodal Microscopy
Integrated multimodal cross-sectional or volumetric
imaging techniques give us fruitful information to understand the
behavior or status of target objects such as biological samples. Most
of the reported systems for this purpose are either time consuming
due to scanning or use additional reference beams such as in
interferometry. Therefore, fast, simple, highly efficient, and powerful multimodal imaging systems that can perform cross-sectional
imaging with simple algorithms are worth to be investigated. In
this paper, a multimodal technique for cross-sectional quantitative
phase and fluorescence imaging with computational microscopy
is presented. We combine cross-sectional fluorescence and quantitative phase imaging by using the transport of intensity equation
(TIE) and numerical wave propagation. The amplitude and phase
of the fluorescence light wave with partially spatial coherence are
obtained from three defocused intensity patterns. The proposed
hybrid imaging system is simple, compact, and non-iterative. We
present experimental results of microbeads and fluorescent proteinlabeled living cells of the moss Physcomitrella patensto demonstrate
the performance of the proposed imaging system
Three-dimensional fluorescence imaging using the transport of intensity equation
We propose a nonscanning three-dimensional (3-D) fluorescence imaging technique using the transport of intensity equation (TIE) and free-space Fresnel propagation. In this imaging technique, a phase distribution corresponding to defocused fluorescence images with a point-light-source-like shape is retrieved by a TIE-based phase retrieval algorithm. From the obtained phase distribution, and its corresponding amplitude distribution, of the defocused fluorescence image, various images at different distances can be reconstructed at the desired plane after Fresnel propagation of the complex wave function. Through the proposed imaging approach, the 3-D fluorescence imaging can be performed in multiple planes. The fluorescence intensity images are captured with the help of an electrically tunable lens; hence, the imaging technique is free from motion artifacts. We present experimental results corresponding to microbeads and a biological sample to demonstrate the proposed 3-D fluorescence imaging technique
Roadmap on holography
From its inception holography has proven an extremely productive and attractive area of research. While specific technical applications give rise to 'hot topics', and three-dimensional (3D) visualisation comes in and out of fashion, the core principals involved continue to lead to exciting innovations in a wide range of areas. We humbly submit that it is impossible, in any journal document of this type, to fully reflect current and potential activity; however, our valiant contributors have produced a series of documents that go no small way to neatly capture progress across a wide range of core activities. As editors we have attempted to spread our net wide in order to illustrate the breadth of international activity. In relation to this we believe we have been at least partially successful.This work was supported by Ministerio de EconomĂa, Industria y Competitividad (Spain) under projects FIS2017-82919-R (MINECO/AEI/FEDER, UE) and FIS2015-66570-P (MINECO/FEDER), and by Generalitat Valenciana (Spain) under project PROMETEO II/2015/015
Media 2: Optical voice recorder by off-axis digital holography
Originally published in Optics Letters on 15 November 2014 (ol-39-22-6549
Media 1: Optical voice recorder by off-axis digital holography
Originally published in Optics Letters on 15 November 2014 (ol-39-22-6549
Media 4: Optical voice recorder by off-axis digital holography
Originally published in Optics Letters on 15 November 2014 (ol-39-22-6549